Office address: Laboratory of Biotechnology
54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand
Tel: +66 25530 8555 (ext. 8369)
E-mail:

Research Scientist

Research Experience (Specialties)
Plant genetic engineering and Molecular genetic in plant

Education
1975-1978 B.Sc. (Plant Pathology) Kasetsart University Thailand
1978-1981 M.Sc. (Microbiology) Kasetsart University Thailand
1988-1991 Ph D. (Virology) Australian NationalUniversity Australia

Awards
2009 Innovative Awards, Kasetsart University
2010 Research Invention Awards, National Research Council of Thailand
2014 Thailand Toray Science Foundation (TTSF)

Training Workshop
1983 Training at Asian Vegetable Development Center, Tainan, Taiwan, on Vegetable diseases for 2 months
1985 Training at Akita Prefectural College of Agriculture, Akita Prefecture, Japan on Monoclonal antibody technics for plant virus research for 2 months
1992 Training at Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Japan on Genomoic study of floricaula gene, FLO (flower tissue specific) of rice and gene transformation of rice using electroporation for 4 moths
2010 Proteomic analysis, Max Planck Institue fro Biology of Ageing, Germany for 5 days

Publications

1. 1. Bhinija, K., Srisomsap, C., Chokchaichamnankit, D., Svasti, J., Mongkolsuk, S., Huehne, P.S. 2017. High resolution proteomic profiles generated from polysaccharide rich tissues of orchids. Planta Maejo International Journal of Science and Technology MIJST-14-46 (In press).
   2. Kaewphalug,W. Huehne, P.S., Sriboonlert, A. 2016. Characterization of CONSTANS-like gene from pigeon orchid (Dendrobium crumenatum Swartz) and its expression under different photoperiod conditions. The Horticulture Journal doi: 10.2503/hortj.MI-123
   3. Pangjai, W., Huehne, P.S. 2015. An analysis of cis-acting regulatory elements related to light response in the 5′ flanking region of the Ascocenda and Dendrobium actin genes Kasetsart Journal (Natural Science) 49:174–187.
   4. Pangjai, W., Huehne, P.S. 2015. The variable amino acid sequences in the S-loop and target binding site of the vegetative actin from the Ascocenda orchid flower  Journal of Plant Biochemistry and Biotechnology 24(4):408–416. Doi:10.1007/s13562-014-0291-5.
   5. Petchthai, U., Anchalee, C., Hunhne, P.S. 2015. Recovery of virus-infected Dendrobium orchids by constitutive expression of the cymbidium mosaic virus coat protein gene. Plant Cell, Tissue and Organ Culture 120:597–606. Doi:10.1007/s11240-014-0626-x.
   6. Petchthai, U., Peng, D., Huehne, P. S. 2015. The bias in small RNA profiles between symptomless Dendrobium and severe symptom Ascocenda orchids infected long-term with Cymbidium mosaic virus. Plant Molecular Biology Reporter (Plant Mol Biol Rep) 33:819–828.  Doi:10.1007/s11105-014-0780-7.
   7. Peyachoknagul, S., Mongkolsiriwatana, C., Wannapinpong, S., Huehne, P.S., Srikulnatha, K. 2014. Identification of native Dendrobium species in Thailand by PCR-RFLP of rDNA-ITS and chloroplast DNA. ScienceAsia 40, 113–120. doi: 10.2306/scienceasia1513-1874.2014.40.113
   8. Patipipat, P., Bhinija, K., Wongkhamprai, B., Hunhne, P. S. 2014. Molecular cloning of arsenate reductase and phytochelatin synthase genes related to arsenic response in Oryza sativa L. ssp. indica cv. Khao Dawk Mali 105. Thai J. Genet. 7(1), 41–50.
   9. Lokkamlue, N., Huehne, P. S. 2013. Molecular Biology of Vanda Miss Joaquim 1-aminocyclopropane-1-carboxylate oxidase gene from the most ethylene-sensitive orchid flower. Maejo International Journal of Science and Technology. 7 (2), 338-352.
   10. Lokkamlue, N., Huehne , P.S. 2013. Sequence Analysis of Ethylene Response Sensor Gene isolated from Vanda Miss Joaquim Flowers. Kasetsart Journal: Natural Science 47(2), 271-284.
   11. Mohamad, Z.A., Chokchaichamnankit, D., Bhinija , K., Monique, N., Paricharttanakul, N. M., Svasti, J., Huehne, P. S., Srisomsap, C. 2013. Proteomic level changes in the root of Brassica alboglabra induced by alachor herbicide. African Journal of Biotechnology 12 (20), 2840-2849.
   12. Jantasuriyarat, C., Ritchuay, S., Pattarawat , P., Huehne, P.S., Kate-Ngam, S,. 2012. Development and transferability of EST-SSR and transferability of genomic SSR markers for genetic diversity assessment of Doritis. Biochemical Systematics and Ecology 45, 57-65.
   13. Huehne, P. S., Bhinija, K. 2012. Application of cryoprotectants to improve low temperature storage survival of orchid seeds. Scientia Horticulterae 135, 186-193.
   14. Wongkhamprai, B., Sangsavang, K. , Sriboonlert, A., Huehne, P. S. 2011. Efficiency of Agrobacterium transformation of pinene synthase gene isolated from kaffir lime in Arabidopsis thaliana. Thai Journal of Genetics 4(2), 85-93.
   15. Mohamad, Z.A., Chokchaichamnankit, D., Bhinija , K., Paricharttanakul, N. M., Svasti, J., Huehne, P. S., Srisomsap, C. 2011. Proteomic analysis of Chinese kale (B. alboglabra) leaves during growth. Journal of Integrated Omics 1, 102-107.
   16. Lertwiriyawong, B., Bhinija, K., Huehne, P.S. 2011. Sequence Analysis of the 16SrRNA–rps12 Inverted Repeat Region in Chloroplast DNA of Dendrobium orchids . Kasetsart Journal – Natural Science 45, 461-72.
   17. Lertwiriyawong, B., Pinthong, K., Chanpaisaeng, J., Saksoong, P., Huehne, P.S. 2010. Analysis of the insecticidal crystal gene type 1 of Bacillus thuringiensis Thai isolates affecting lepidopterans. ScienceAsia 36, 271–279. doi: 10.2306/scienceasia1513-1874.2010.36.271
   18. Sintuprapa, W., Theeragool, G., Yongmanitchai, W., Huehne, P.S., Matsushita, K. 2008 Molecular taxonomy of acetobacter syzygii SKU19 and characterization of its acetic acid adapted strains. Kasetsart Journal – Natural Science 42, 701-14.
   19. Suwanaketchanatit, C., Piluek, J., Peyachoknagul, S., Huehne, P.S. 2007. High efficiency of stable genetic transformation in Dendrobium orchid via microprojectile bombardment. Biologia Plantarum 51, 720-727.
   20. Suwanaketchanatit, C., Chaisuk, P., Piluek, J., Peyachoknagul, S., Huehne, P.S. 2006. Evaluation of constitutive promoters for gene expression in Dendrobium protocorms and flowers. Kasetsart Journal (Nat. Sci.) 40, 934-943.
   21. Huehne, P.S., Rungroj N., Keeratiyaangul, S. 2006. Identification of the conserved sequences at the species level of odontoglossum ringspot virus and other members of tobamoviruses by its coat protein sequence analysis. Kasetsart Journal (Nat. Sci.) 40(2), 376-387.
   22. Huehne, P.S., Eurjirapongpun, P., Jampatong, C. 2006. Transformation of chitinase gene in Thai sweet corn inbred lines using particle bombardment and Agrobacterium. Agriculture Sci. J. 37(4), 375-384.
   23. Bhinija, K., Ruengverayut, S., Loprasert, S., Huehne, P.S. 2004. Development of a minimal medium to prolong the subculturing period of Vanda, dendrobium and Coelogyne orchids in vitro. Agriculture Sci. J. 35(3-4), 97-103.
   24. Arayaskul, N, Srifah, P., Piluek, C. 2003. Detection of potexvirus and tobamovirus causing diseases in orchids. Agriculture Sci. J. 33(4-5), 173-186.
   25. Adams, R.P., Zhong, M., Srifah, P., Sanduen, N. 1999. DNA Genetics Diversity and Systematics of Vetiveria zizanioides (Poaceae). Phytologia 85, 85-95.
   26. Srifah, P., Loprasert, S., Rungrol, N. 1996. Use of Reverse Transcription-Polymerase Chain Reaction for Cloning of Coat Protein-encoding Genes of Cymbidium Mosaic Virus. Gene. 179, 105-107.
   27. Srifah, P., KEESE, P., Weiller, G., Gibbs, A. 1992. Comparisons of the Genomic Sequences of Erysimum Latent Virus and other Tymoviruses. Journal of General Virology. 73, 1437-1447.
   28. Gibbs, A., Gibbs, J., Mackenzie, A., Mo, J.S., Osorio-Keese, M., Srifah, P., Torronen, M., Weiller, G. 1991 The molecular phylogenetic of the virion protein of tymoviruses. Virology
   29. Ding, S, Howe, J, Keese, P., Mackenzie, A., Skotnicki, M., Srifah, P., Torronen, M., Gibbs, A. 1990. The Tymobox, a sequence shared by most tymoviruses: its use in molecular studies of Tymoviruses. Nucleic Acids Research. 18, 1181-1187.
   30. Gibbs, A., Ding, S, Howe, J, Keese, P., Mackenzie, A., Skotnicki, M., Srifah, P., Torronen, M. 1990. Old versus new characters for systematic: Cautionary tales from virology. Aust. Syst. Bot. 3, 159-163.

Srifah, P., Keeses, P., Shukla, D., Gibbs, A. 1990. The Primary Structure of the Virion Protien Gene and Encoded Protien of Erysimum Latent Tymovirus. Journal of General Virology. 71, 3085-3088.