Dr. Voraratt Champattanachai


voraratt
Name: Dr. Voraratt Champattanachai

Office address: B705A, 7th Floor, Biomedical Science Building
54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand
Tel: +66 25530 8555 (ext. 8347 )
E-mail: voraratt 

Research Scientist I

Research Interests
My research has been focused on abnormal proteins associated to diseases including inborn error of metabolism (IEM) and cancer. IEMs are rare diseases found in children; however, more than 500 types currently identify around the world. They normally result from defects in a single gene coding for an enzyme in living metabolism. In collaboration with pediatricians, the objective of this research is to reveal unusual inborn errors of metabolism not previously characterized in Thailand and to study the molecular mechanisms by which they lead to their defects as well as to elucidate the correlation between genotype and phenotype of diseases.

The second project is to search novel cancer biomarker. This research aims to identify and characterize post-translational protein modifications mainly in O-GlcNAcylation. This glycosylation is thought to be a nutrient sensor in highly proliferating cells via the hexosamine biosynthesis pathway, a minor branch of glycolysis. O-GlcNAcylation is a reversible reaction of the attachement of single N-acetylglucosamine (GlcNAc) molecule to serine and threonine residues which is similar to phosphorylation. Understanding of proteins modified this glycosylation may provide precise mechanisms of cancer biology and O-GlcNAc modified proteins identified may be novel biomarkers of cancer.
Many laboratory techniques will be used for both researches including proteomic 2-D IEF/PAGE, immunoblotting, immunoprecipitation, mass spectrometer analysis, molecular techniques, RNA interference (RNAi), bacterial and mammalian cell culture techniques, HPLC, DNA sequencing, recombinant protein expression and fluorescent spectrophotometer and microscope.

Education
B.Sc. (Biology), Kasetsart University, Thailand
M.S. (Biochemistry), Mahidol University, Thailand
Ph.D. (Cell Biology),The University of Alabama at Birmingham, U.S.A.

Publications
1. Srisomsap C, Svasti J, Surarit R, Champattanachai V, Sawangareetrakul P, Boonpuan K, Subhasitanont P, and Chokchaichamnankit D. Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre. J Biochem (Tokyo) 119: 585-590, 1996.
2. Srisomsap C, Svasti J, Surarit R, Champattanachai V, Sawangareetrakul P, Boonpuan K, Subhasitanont P, and Chokchaichamnankit D. Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre. J Biochem (Tokyo) 119: 585-590, 1996.
3. Ketudat Cairns JR, Champattanachai V, Srisomsap C, Wittman-Liebold B, Thiede B, and Svasti J. Sequence and expression of Thai Rosewood beta-glucosidase/beta-fucosidase, a family 1 glycosyl hydrolase glycoprotein. J Biochem (Tokyo) 128: 999-1008, 2000.
4. Champattanachai V, Ketudat Cairns JR, Shotelersuk V, Keeratichamroen S, Sawangareetrakul P, Srisomsap C, Kaewpaluek V, and Svasti J. Novel mutations in a Thai patient with methylmalonic acidemia. Mol Genet Metab 79: 300-302, 2003.
5. Ketudat Cairns JR, Keeratichamroen S, Sukcharoen S, Champattanachai V, Ngiwsara L, Lirdprapamongkol K, Liammongkolkul S, Srisomsap C, Surarit R, Wasant P, and Svasti J. The molecular basis of mucopolysaccharidosis type I in two Thai patients. Southeast Asian J Trop Med Public Health 36: 1308-1312, 2005.
6. Nagy T, Champattanachai V, Marchase RB, and Chatham JC. Glucosamine inhibits angiotensin II-induced cytoplasmic Ca2+ elevation in neonatal cardiomyocytes via protein-associated O-linked N-acetylglucosamine. Am J Physiol Cell Physiol 290: C57-65, 2006.
7. Champattanachai V, Marchase RB, and Chatham JC. Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein-associated O-GlcNAc. Am J Physiol Cell Physiol 292: C178-187, 2007.
8. Keeratichamroen S, Cairns JR, Sawangareetrakul P, Liammongkolkul S, Champattanachai V, Srisomsap C, Kamolsilp M, Wasant P, and Svasti J. Novel mutations found in two genes of thai patients with isolated methylmalonic acidemia. Biochem Genet 45: 421-430, 2007.
9. Champattanachai V, Marchase RB, and Chatham JC. Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and mitochondrial Bcl-2. Am J Physiol Cell Physiol, 294(6): C1509-20, 2008.
10. Zou L, Yang S, Champattanachai V, Hu S, Chaudry IH, Marchase RB, Chatham JC. Glucosamine improves cardiac function following trauma-hemorrhage by increased protein O-GlcNAcylation and attenuation of NF-(kappa)B signaling. Am J Physiol Heart Circ Physiol. 296(2): H515-23, 2009.
11. Vatanavicharn N, Champattanachai V, Liammongkokul S, Sawangareetrakul P, Keeratichamroem S, Cairns J, et al. Genetic analysis of mut, MMAA, and MMAB in Thai patients with isolated methylmalonic acidemia. Journal of Inherited Metabolic Disease [BC]. 2010; 33 (supplement: Suppl. (AUG 2010)): S42-S.
12. Vatanavicharn N, Champattanachai V, Liammongkolkul S, Sawangareetrakul P, Keeratichamroen S, Ketudat Cairns JR, Srisomsap C, Sathienkijkanchai A, Shotelersuk V, Kamolsilp M, Wattanasirichaigoon D, Svasti J, Wasant P., Clinical and molecular findings in Thai patients with isolated methylmalonic academia. Mol Genet Metab. 106(4): 424-9, 2012.
13. Charoenwattanasatien R, Cairns JR, Keeratichamroen S, Sawangareetrakul P, Tanpaiboon P, Wattanasirichaigoon D, Pangkanon S, Svasti J, Champattanachai V., Decreasing Activity and Altered Protein Processing of Human Iduronate-2-sulfatase Mutations Demonstrated by Expression in COS7 Cells. Biochem Genet. 50: 990-7, 2012.
14. Champattanachai V, Netsirisawan P, Chaiyawat P, Phueaouan T, Charoenwattanasatien R, Chokchaichamnankit D, Punyarit P, Srisomsap C, Svasti J. Proteomic analysis and abrogated expression of O-GlcNAcylated proteins associated with primary breast cancer. Proteomics. 13(14): 2088-99, 2013.
15. Phueaouan T, Chaiyawat P, Netsirisawan P, Chokchaichamnankit D, Punyarit P, Srisomsap C, Svasti J, Champattanachai V. Aberrant O-GlcnAc-modified proteins expressed in primary colorectal cancer. Oncology Reports. 30: 2929-2936, 2013

 

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